The preparation of chitosan-based nanoparticles by ionotrophic gelation often leads to high batch-to-batch variability as well as in low reproducibility. A possible solution to this could be controlled mixing on a microfluidic scale. In the study presented here, chitosan tripolyphosphate nanoparticles with siRNA, mRNA and β-galactosidase as cargo will be prepared using microfluidics.
More than 5 million people worldwide are affected by inflammatory bowel disease. To enable good treatment, targeted and efficient drug delivery is necessary. In the presented study, a chitosan-based vancomycin loaded microgel was investigated for this purpose.
In microfluidic systems, analyses can be performed on a miniaturized scale without the use of complex laboratory equipment. In this context, DNA purification and concentration pose a particular challenge. To improve this, a disposable microfluidic device is functionalized with different chitosans for DNA binding in the presented study. This will then be used to detect eDNA of invasive species from river water samples.