News

head uk2

Chitosan-coated selenium nanoparticles for brain cancer therapy

Glioblastomas are difficult to treat due to the blood-brain barrier and drug resistance. To improve the efficacy of treating glioblastomas with selenium nanoparticles (Se-NPs), Se-NPs were coated with chitosan in the presented study.

Tags: chitosan, nanoparticles, Selenium, glioblastoma

INHIBITING METASTASIS AND IMPROVING CHEMOSENSITIVITY VIA CHITOSAN-COATED SELENIUM NANOPARTICLES FOR BRAIN CANCER THERAPY

Dana, P.; Pimpha, N.; Chaipuang, A.; Thumrongsiri, N.; Tanyapanyachon, P.; Taweechaipaisankul, A.; Chonniyom, W.; Watcharadulyarat, N.; Sathornsumetee, S.; Saengkrit, N. Inhibiting metastasis and improving chemosensitivity via chitosan-coated selenium nanoparticles for brain cancer therapy. Nanomaterials 2022, 12, 2606. https://doi.org/10.3390/nano12152606

Glioblastoma is the most common and aggressive type of malignant brain tumor in adults. Besides a poor quality of life, the chances of survival are low. This is due to problems in treatment, on the one hand drug resistance occurs, on the other hand only about 1 % of the drug reaches its destination due to the blood-brain barrier. For this reason, new strategies are urgently needed to enable more efficient treatment.

Selenium (Se) is an essential element needed by the body to maintain its functions. Due to its pro-oxidant properties, Se is used as an adjuvant in chemo- and radiotherapy of e.g. liver, breast or brain cancer. However, Se is toxic in high doses and its uptake is difficult to regulate.

To reduce toxicity and improve biocompatibility, Se can be bound to nanoparticles (NPs). In vivo and in vitro, SeNPs have already been shown to have good efficiency and tolerable toxicity. To further improve the stability and therapeutic effect of SeNPs, the surface of these can be optimized with polymers such as chitosan.

In the presented study, the inhibitory effect of chitosan-coated SeNPs on the aggressiveness of GBM cells in terms of cell proliferation, migration and invasion is investigated in a 3D spheroid tumor model.  

RESULTS

  • CS-SeNPs showed significantly reduced particle diameter at 88.66 ± 0.65 nm, 88.90 ± 0.57 nm, and 91.45 ± 2.57 nm compared to Se-NPs (417.60 ± 46.17 nm)
  • Reduced PDI between 0.2±0.01 and 0.23±0.02 (SeNPs: 0.48 ± 0.08) indicates improved particle stability
  • Stable particle size and zeta potential of more than 30 mV over 15 days.
  • 2% CS-SeNPs showed great selectivity between U87 cells (glioblastoma cells) and fibroblasts (healthy cells) with the lowest cell viability of U87 cells and at the same time the lowest cytotoxicity to fibroblasts
  • Improvement of the sensitivity of U87 cells to the chemotherapeutic agent 5-fluorouracil by 0.2% CS-SeNPs
  • Inhibition of cell migration and cell invasion of glioblastoma cells by inhibition of MMP-2/9 activities
  • Enhancement of cellular uptake of CS-SeNPs in U87 cells by chitosan coating.
  • In vitro evidence that CS-SeNPs are potentially able to cross the blood-brain barrier

Summary: In the presented study, CS-SeNPs were successfully synthesized, which exhibited improved stability and smaller size compared to SeNPs. Moreover, 0.2% CS-SeNPs in particular showed improved antitumor activity against glioblastoma cells with only minor effects on fibroblasts. In an in vitro model, possible transport across the blood-brain barrier was demonstrated, which is essential for more efficient treatment of glioblastoma. To further assess the potential of CS-SeNPs further experiments in vivo have to be performed.

Link to article: Nanomaterials | Free Full-Text | Inhibiting Metastasis and Improving Chemosensitivity via Chitosan-Coated Selenium Nanoparticles for Brain Cancer Therapy (mdpi.com)

Chitosans of different specifications can be found in our shop. If you have any questions, do not hesitate to contact us by mail.

Congress and fairs

Meet us in person 2022:

  • EUCHIS, 2022
  • CPHI 2022
  • Medica 2022
  • Asia Pacific Chitin and Chitosan Symposium, 2022, South Korea

To arrange an appointment please contact Katja Richter via sales(at)medical-chitosan.com

Contact

  • Heppe Medical Chitosan GmbH
    Heinrich-Damerow-Strasse 1
    06120 Halle (Saale)
    Germany
  • Tel.: +49 (0) 345 27 996 300
    Fax: +49 (0) 345 27 996 378
  • sales@medical-chitosan.com

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.