Christian Augsten, Karsten Mäder
Flow Field-Flow Fractionation (Flow-FFF) is a powerful tool to separate molecules and particles in a range from 103 to 107Da and particles of nanometric size up to a few micrometers . By coupling FFF to Multi-angle light scattering (MALS) it is possible to determine absolute molar masses (M) of molecules and to verify their aggregation. For macromolecules of an appropriate size the radius of gyration (rg) can be achieved . The aim of our study was to investigate various chitosan types by applying Flow-FFF and MALS to their aqueous solutions.